DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


Contact Us (02) 4339 1029 sales@dwscientific.com.au

Follow Don Whitley Scientific Pty Ltd

News

WASP Touch Spiral Plater

New WASP Touch Spiral Plater Video

Don Whitley Scientific is pleased to announce a new video featuring WASP Touch, the spiral plater designed for the needs of modern microbiology laboratories. The video outlines the way in which spiral plating brings several cost and time savings to the laboratory, as well as discussing the options and accessories that are available to help tailor the system to your particular application.

If you haven’t already seen WASP Touch, here’s some background information on the product. If you need any further information, please see our website or contact us at sales@dwscientific.co.uk.

Read more

Clostridium difficile studies can be done in a Whitley Workstation

New Whitley Workstation Videos

 

Not convinced you need an anaerobic workstation?

Still juggling jars to grow your anaerobes?

Need the perfect atmosphere for your microaerophiles?

 

These new videos from Don Whitley Scientific will make you think again and realise that you can do so much more with a workstation:

An Introduction to Whitley Anaerobic Workstations

An Introduction to Whitley Microaerobic Workstations

As well as introducing the range of workstations available, the videos give an insight into the options and accessories that can tailor your anaerobic or microaerobic cabinet to your particular application or study.

You may work in a busy clinical or contract laboratory with a high throughput of samples to process. You could be a researcher performing a range of experiments, restricted by the limitations of the equipment available to you. Whatever your discipline, let us show you how you can benefit from a Whitley Workstation.

For further information, please contact our UK sales team on 01274 595728. For visitors from other countries, please contact your local distributor from the list on our website.

 

Samantha Fryer being presented with her award

Leeds University Poster Prizes 2017

Frank Charlton - 2nd Prize - Lab based projects

Frank Charlton – 2nd Prize – Lab based projects

Andrew Pridmore, Head of Microbiology, visited the University of Leeds Faculty of Biological Sciences on 4th May 2017 to present prizes to undergraduate students on the Microbiology, Medical Microbiology and Microbiology in relation to Medicine BSc degree courses.

These prizes, sponsored by Don Whitley Scientific, are awarded each year for poster presentations of the students’ final year research projects.  These are divided into two categories – laboratory research and literature review.  The winners this year were:

Laboratory-based projects:

1st   –    Samantha Fryer: “Lifestyle Choices of Dietary Supplement Users” (Samantha is pictured top left with Dr Pridmore)

2nd  –    Frank Charlton: “A Pharmacological Approach to Identifying BUNV Entry Mechanisms”

3rd   –    Danielle Beeson: “Mutant calreticulin requires Tyr-626 of the thrombopoietin receptor for oncogenic transformation”

Literature reviews:

Prize winner Katherine Kelleher with Dr Andrew Pridmore

Prize winner Katherine Kelleher with Dr Andrew Pridmore

1st   –    Katherine Kelleher: “How to create a successful oncolytic agent”

2nd  –    Kurt Rushworth: “Brain Metastases: Mechanisms and Therapeutics”

3rd   –    Hope Denyer: “Old Drugs, New Wheels: reinvigorating natural products against cancer”

All posters were of a very high standard, but Andrew was especially pleased to find several research projects on the subject of antibiotic susceptibility / resistance and some interesting concepts for novel antibacterial therapies.  A selection of these is provided below:

  • Adhiron and the antibiotic resistance crisis: a change in direction (Edward Davies)
  • Can understanding bacterial immune evasion strategies help to generate novel antivirulence therapeutics? (Rebecca Golenya)
  • Immune evasion strategies employed by Pseudomonas aeruginosa as potential drug targets (Marie Horsler)
  • BAM: A solution to the antibiotic resistance crisis in Gram-negative bacteria? (Charles Kelleher)
  • The emerging role of anti-virulence therapies within antimicrobial resistance (Scarlet-Daisy Prior)

We wish the very best of luck to all of the final year students who are now immersed in their examinations!

To download copies of any of the above papers, please go to this page on our website.

 

CHROMAZONA Automatic Colony Counter

BSMT Annual Scientific Conference

Don Whitley Scientific will be exhibiting at the British Society for Microbial Testing annual conference, held on 12th May at Public Health England in Colindale. 

The BSMT conference is aimed at senior biomedical and clinical scientists, other scientists and medical microbiologists. The meeting will feature talks on a wide range of relevant microbiology topics. The programme features UK and international speakers, who will deliver a range of talks on the day. Don Whitley Scientific will be there to exhibit at the event. Sales representatives will be on hand to discuss how our product range can benefit a range of microbiology applications.

The featured product on the Don Whitley Scientific exhibition stand will be the ChromaZona, an automated microbial identification and antibiotic susceptibility testing (AST) system, which provides faster results for busy laboratories. ChromaZona features automatic comparison with EUCAST MIC breakpoint values and provides fully traceable information for UKAS accreditation.

Also on the Don Whitley Scientific stand is the Whitley Interactive Product Presentation. This interactive display will give delegates the option to explore the full range of Whitley Workstations and access in-depth information on each product. Make sure to visit the Don Whitley Scientific exhibition stand if you are attending this event.

jane-freeman-thumbnail

Why Choose a Whitley Workstation?

There are many reasons to choose a Whitley Workstation when it comes to Anaerobic, Hypoxic or Microaerophilic work. We can discuss these with you anytime, but we also have plenty of satisfied customers who have expressed why using a Whitley Workstation improves their working methods and results.

Over the years, customers have supplied us with many testimonials about their Don Whitley Scientific products. From these we can see that not only have Whitley Workstations become approved by fantastic researchers worldwide, but we can also help promote the amazing work that is done by our customers.

Dr Vaibhao Janbandhu at the Victor Chang Cardiac Research Institute (VCCR) in Sydney, Australia uses a Whitley H35 Hypoxystation in his work on finding new ways to stimulate heart regeneration during ageing and after heart attack. He uses his H35 Hypoxystation to isolate, culture and characterise adult cardiac stem cells. In Dr Janbandhu’s words the H35 is “an integral part of the project to advance the project aims”.

In this video testimonial, Jane Freeman at Leeds General Infirmary explains how her Whitley A95 Workstation improves the working methods in her Clostridium difficile research. Jane reports that she and her team are able to use the workstation for “several hours at a time in relative comfort” and that the workstation is able to house all the technical equipment her team requires. This allows “the whole experiment to be performed in optimum conditions without introducing air at all”. Jane explains that “reliability, versatility and space are the significant benefits of the workstations in our work on Clostridium difficile“.

The Institute of Cancer Research in London is one of the world’s most influential research institutes, with an outstanding record of achievement dating back more than 100 years. At the Institute, George Poulogiannis uses a combination of Whitley i2 Instrument Workstation (with Seahorse XF Analyzer) and Whitley H35 Hypoxystation in his research into breast cancer. Hypoxia is a key factor in the “Hallmarks of Cancer” and this team are studying the role of hypoxia in cell invasion and metastasis, oncogene-induced senescence and resistance to current treatment options. The i2 and H35 replicate a physiologically relevant atmosphere for these studies, enabling consistent and reliable results. This combination of Workstations is also used by Dr Ayse Latif, who is researching gynaecological cancers at The University of Manchester.

Don Whitley Scientific would like to take this opportunity to thank all customers who have provided testimonials. If you would be interested in supplying a testimonial, please contact Alex_Rhodes@dwscientific.co.uk.

Take a look at our other testimonials

capture

HypOxygen at Tumour Microenvironment Workshop in Miami

This article was written by Burga Kalz Fuller, join her and HypOxygen at the 15th International Tumour Microenvironment Workshop in Miami

In most cancers, the hypoxic microenvironment affects the development and progression of tumours, driving alterations in gene expression, metabolism and cell signalling, and significantly influencing the Hallmarks of Cancer. So what about in vitro cancer research, do culture parameters matter? Definitely! Numerous studies have shown that even very brief exposure to ambient oxygen levels and temperature significantly impacts cell culture, behaviour and function of cells in vitro.

HypOxygen will be exhibiting our Hypoxystation at the 15th International Tumour Microenvironment Workshop in Miami from 27th – 29th April. The special focus there is on “Hypoxia, Angiogenesis and Vasculature”, reflecting the critical importance of hypoxia in the context of cancer. With the Hypoxystation, cancer researchers have their finger on the pulse of physiological cell culture.

 

hypoxia

 

The Hypoxystation mimics the hypoxic conditions present in cancer, providing a closed workstation format with contiguous, stable low oxygen down to 0.1%. Precise oxygen, carbon dioxide, and humidity control within a temperature-controlled environment, as well as ample space for cellular manipulation, assays and microscopic observation, allow researchers to recreate physiological conditions. HEPA filtration, sterile steam humidification, and remote parameter monitoring are some of the features that make the Hypoxystation so unique.

Cancer research labs, who use a Hypoxystation to re-create hypoxic conditions in the tumour microenvironment, are publishing brilliant papers which demonstrate the influence of hypoxia on the Hallmarks of Cancer. Metabolic adaptation, sustained growth, resisting cell death, and angiogenesis are just some of the Hallmarks which are affected by hypoxia. Here are some recent highlights:

Hypoxystation users are showing that “culturing cells in ambient air, or ‘normoxia’ is far from physiological.

Visit HypOxygen at the 15th International Tumor Microenvironment Workshop in Miami

eccmid logo

See the WASPLab at ECCMID 2017 in Vienna

WASPLab to be shown at the forthcoming 27th European Congress of Clinical Microbiology and Infectious Diseases.

Held in Vienna, this event brings together the world’s leading experts to discuss the latest developments in infectious diseases, infection control and clinical microbiology. The scientific programme features talks from key figures in antibiotic susceptibility testing, infection control and antimicrobial resistance.

Representatives from Don Whitley Scientific will be present on the Copan stand at this event to help promote WASPLab. WASPLab is the sophisticated barcode driven microbiology specimen processor and work-up system, moving samples from front end processing to full specimen management, automated incubation and digital microbiology.  With its modular design and small footprint, WASPLab can be customised to the unique needs of the lab.  The robotic plate management system, smart incubators and state-of-the-art image acquisition technology are changing the way labs work and opening the door for groundbreaking digital microbiology.

Don Whitley Scientific is one of the primary distributors of the WASPLab in the UK. We have installed a WASPLab at Leeds General Infirmary and will soon be installing a second system at Manchester Royal Infirmary. We invite you to come along and meet representatives from Don Whitley Scientific on Copan’s stand at this international event.

 

WASPLab

WASPLab

Ji Zhang presenting his poster at the Keystone Symposia

Hypoxia and Tumour Metabolism in Whistler with HypOxygen

This article was written by Burga Kalz Fuller of HypOxygen, giving an account of her and HypOxygen’s recent involvement at the Keystone meeting in Whistler, Canada. 

Really, a day in Whistler doesn’t get any better: talks on the newest results on hypoxia and tumour metabolism from morning till night, and outside the snow falls all day, every day. The joint Keystone Symposia on “Adaptations to Hypoxia in Physiology and Disease” and “Tumour Metabolism: Mechanisms and Targets” in Whistler, British Columbia last week featured both skiing and science, and HypOxygen was honoured to be a part of it all.

Joint sessions every day highlighted the many ways in which hypoxia controls gene expression, influences metabolic pathways, and regulates immunological and inflammatory processes, with new data showing how hypoxia affects the Hallmarks of Cancer. North American Hypoxystation users Navdeep Chandel, Nick Denko and Brad Wouters gave talks on respiration, mitochondrial function, and hypoxic regulation of autophagy. European Hypoxystation users Almut Schulze, Janine Erler and Ester Hammond spoke about glucose/lipid metabolism, ECM remodeling and DNA replication in hypoxia. Together, a global community of cancer researchers are targeting hypoxia as a key factor underlying tumour genesis and cancer progression.

Some of our own Hypoxystation users gave poster presentations: Ji Zhang (pictured top left) from Brad Wouters’ lab at Princess Margaret Cancer Center had a poster on “Characterizing oxygen metabolism and hypoxia tolerance in pancreatic ductal adenocarcinoma“, and Sara Timpano from Dr. Jim Uniacke’s lab at University of Guelph presented “Investigating cellular metabolism, DNA damage, and oxidative stress response under physiological oxygen conditions“. Hypoxystation users Navdeep Chandel, Nick Denko and Brad Wouters gave talks on respiration, mitochondrial function, and hypoxic regulation of autophagy, to name just a few.

 

Sarah Timpano presenting her poster at the Keystone Symposia

Sarah Timpano presenting her poster at the Keystone Symposia

We spoke to many of the Keystone attendees about our Whitley i2 Instrument Workstation and the Whitley H35 HEPA Hypoxystation by Don Whitley Scientific. The closed workstation format of the Hypoxystation provides reliable hypoxia down to 0.1% for cells accustomed to the very low oxygen customary in any body tissue, and especially in the tumour microenvironment. Precise oxygen, carbon dioxide, and humidity control within a temperature-controlled environment as well as ample space for cellular manipulation, assays and microscopic observation allow researchers to mimic and monitor physiological conditions. HEPA filtration, sterile steam humidification, and remote parameter monitoring are some of the features that make the Hypoxystation so unique.

As Jim Uniacke states in this video tutorial on creating physiological oxygen, “It is important to keep cells in the hypoxia workstation up until the point of lysis, as oxygen can rapidly alter the biochemical properties of these translation factors.” His lab has been producing exciting results on translation control at hypoxia with the Hypoxystation for several years, earning him the honorary title of “cancer cells’ worst nightmare.” Dr. Uniacke and all the other researchers at the Keystone symposia are working on conquering the nightmare of cancer, Hypoxygen and Don Whitley Scientific want to assist you in that endeavour where possible.

 

sma spring meeting

Scottish Microbiology Association Spring Meeting

Don Whitley Scientific recently headed up north into the Cairngorms National Park to attend the Scottish Microbiology Association Spring Meeting at the Macdonald Resort in Aviemore. 

The meeting featured talks and presentations from various different people working in microbiology in Scotland, with topics ranging from antimicrobial resistance to invasive infections. The event gave Don Whitley Scientific a chance to interact and network with key figures in Scottish microbiology and discuss how the DWS product range may benefit their working methods.

Don Whitley Scientific had an A35 Anaerobic Workstation on their stand, equipped with many unique options and features. Many microbiology labs in Scotland are well aware of Whitley Workstations, already using them in their labs, however the meeting was a good opportunity to introduce the product to those who were looking for a more efficient and easy way to cultivate anaerobes.

sma spring meeting 2

Cairngorms National Park

Find out more about the Don Whitley Scientific range of Anaerobic Workstations

Discover how the Whitley A95 Anaerobic Workstation is optimising conditions for Clostridium difficile

A35_Front_June2014_1000x1000

Cultivating the “Unculturables”

The oral bacteriome comprises about 700 species, most of them anaerobic and participating in symbiotic relationships with their human host and each other which are essential for overall health, not just of the mouth but also of the heart, the brain, and other organ systems. Up to one third of these bacteria have been characterised solely by culture-independent molecular methods such as 16S rRNA cloning, but have yet to be cultivated in vitro. These bacteria are so difficult to culture outside of their biofilm habitat because they rely on metabolic cooperation and intercellular signalling with the community.

Sonia Vartoukian and William Wade of Queen Mary University of London, using their Don Whitley Scientific Anaerobic Workstations, have been shining a bright light into the dark niches of the oral cavity for years. They have identified a novel species in a new genus, Fretibacterium fastidiosum, through co-culture with other oral bacteria cultured in the anaerobic workstation. More recently, they were able to isolate five novel strains from subgingival plaque, using a combination of community culture with helper strains and supplementation with siderophores as growth supplements. The bacteria are surprisingly agile in adapting to changes in their co-dependent habitat, as long as they are provided with the signals and factors they themselves have lost the ability to synthesize. Over the course of up to 21 day culture of the samples, Vartoukian and Wade were fastidious about not exposing the cultures to air, using plates that were pre-reduced in the workstation’s anaerobic atmosphere and making sure to minimise time spent outside of the workstation. The Whitley Anaerobic Workstation makes it easy to work with sensitive cultures. The 10mm thick annealed acrylic, patented use of Anotox, rapid transfer airlock, and easy-to-use sleeve gassing system ensure a robust and strictly anaerobic atmosphere.

 

Image from the website of Yihong Li of the Department of Dentistry, NYU

Image from the website of Yihong Li of the Department of Dentistry, NYU

 

On the other side of the Atlantic, Microbiology International distributes the Don Whitley Scientific anaerobic workstation to North American groups researching the oral bacteriome in physiology and disease. Dr. Yihong Li at New York University Department of Dentistry uses his A35 workstation “to facilitate cutting-edge research in clinical microbiology, antimicrobial treatment evaluation, and infectious disease identification.” The A35 can accommodate up to 600 90 mm plates and features bare-handed access to a consistent and strictly anaerobic environment, reliably monitored by the Anaerobic Conditions Monitoring System. Dr. Li’s research on dental caries has shown that the anaerobic environment is essential for colonization by oral lactobacilli. His group’s large-scale studies of the diversity of lactobacilli associated with severe early childhood caries have demonstrated the necessity to provide a range of anaerobic and microaerophilic niche environments in order to capture the complexity of Lactobacillus variables.

READ MORE

Dr. Li’s group will be presenting new research on oral biofilms at the AADR conference.

Visit Microbiology International at  the AADR/IADR meeting in San Francisco on 22-25 March to experience anaerobic workstations for yourself!