Whitley H135 HEPA Hypoxystation

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Whitley H135 HEPA Hypoxystation

Perfect for accommodating items of laboratory equipment

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The H135 HEPA is a tall, wide, deep hypoxic chamber. This Hypoxystation has a capacity of 900 litres and can accommodate a variety of items of equipment such as live cell imaging devices, microscopes, plate readers, etc.

The generous internal height facilitates easy pipetting. All cell manipulations can be performed inside the chamber's hypoxic conditions instead of removing them and risking cell stress and contamination. The entire cell culture incubator chamber is a working and incubation space.

This workstation provides precise environmental control whilst leaving plenty of room to work, incubate and conduct analysis. A rapid 16 litre airlock – no risk of compromising conditions inside the chamber (and it’s flushed with nitrogen as an additional cost saving).

If you need to aspirate biological liquid waste from inside the chamber, order with a Vacuum Take-off Port and the Whitley AsPROvac for the perfect package.

For those who want to use a Seahorse XF Analyzer in their work, there is the option of connecting the H135 HEPA to a Whitley i2 Instrument Workstation.

Equilibrate your media

Although the Hypoxystation allows tight control of the conditions under which cells are cultured, cells may still be unintentionally exposed to excess oxygen during media changes if the fresh media has not previously been equilibrated with the incubation atmosphere. With the Whitley Media Conditioner (UK Patent No. GB2579704)  you can equilibrate your liquid media rapidly and ensure even greater accuracy in your research. 

  • Precise control of oxygen, carbon dioxide and nitrogen – provides flexibility in your research and confidence in your results.
  • The Whitley Internal HEPA Filtration System with optional Enhanced Biological Containment. 
  • A removable front (fitted with two patented oval ports) to facilitate the transfer of large items of equipment for use inside the chamber.
  • Innovative full colour touch screen that is Ethernet-enabled for remote access. Now you can log into your workstation when you are away from the laboratory.
  • Fully automated oxygen calibration routine (Patent No. 3109635) – activated via the touchscreen with no need to touch or remove the sensor - saves time, no risk of user error and ensures the accuracy of your results.
  • Options Include the Whitley Automatic Humidifier – sterile, on-demand moisture addition. 

View Specification


Capacity (litres)
Airlock Capacity
Accommodates up to 44 x 96 well plates or 84 x T25 flasks
Airlock Size (litres)
Porthole System
Manual, oval ports
Gas Supplies
CO2 / N2 / Air
Auto Sleeve Gassing
Internal Mains Sockets
Storage Tray
Inspection Lamp
Single Sample Entry
Data Logging
Airlock Cycle Time (seconds)
Extra Cable Glands
Contact Us
Automatic Dehumidifier
Automatic Humidifier
Removable Front
Trolley Available
CO2 Monitoring
Vacuum Take Off
Temperature Range
4°C above ambient up to 45°C
Touchscreen Control
Remote Access
As Standard
Dimensions (W/D/H mm)
1473 / 1056 / 1063
Weight (lbs/kgs)
386 / 175

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If you are based in Germany or Australia, please select the website of our subsidiary company there. A different selection of products is available in these countries. Otherwise, please select ‘English’ and you will be able to find your local distributor in the ‘Overseas Distributors’ section.

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