This article was written by Burga Kalz Fuller, join her and HypOxygen at the 15th International Tumour Microenvironment Workshop in Miami
In most cancers, the hypoxic microenvironment affects the development and progression of tumours, driving alterations in gene expression, metabolism and cell signalling, and significantly influencing the Hallmarks of Cancer. So what about in vitro cancer research, do culture parameters matter? Definitely! Numerous studies have shown that even very brief exposure to ambient oxygen levels and temperature significantly impacts cell culture, behaviour and function of cells in vitro.
HypOxygen will be exhibiting our Hypoxystation at the 15th International Tumour Microenvironment Workshop in Miami from 27th – 29th April. The special focus there is on “Hypoxia, Angiogenesis and Vasculature”, reflecting the critical importance of hypoxia in the context of cancer. With the Hypoxystation, cancer researchers have their finger on the pulse of physiological cell culture.
The Hypoxystation mimics the hypoxic conditions present in cancer, providing a closed workstation format with contiguous, stable low oxygen down to 0.1%. Precise oxygen, carbon dioxide, and humidity control within a temperature-controlled environment, as well as ample space for cellular manipulation, assays and microscopic observation, allow researchers to recreate physiological conditions. HEPA filtration, sterile steam humidification, and remote parameter monitoring are some of the features that make the Hypoxystation so unique.
Cancer research labs, who use a Hypoxystation to re-create hypoxic conditions in the tumour microenvironment, are publishing brilliant papers which demonstrate the influence of hypoxia on the Hallmarks of Cancer. Metabolic adaptation, sustained growth, resisting cell death, and angiogenesis are just some of the Hallmarks which are affected by hypoxia. Here are some recent highlights:
- Eales et al. (2016) Hypoxia and metabolic adaptation of cancer cells
- Prickaerts et al. (2016) Hypoxia increases genome-wide bivalent epigenetic marking by specific gain of H3K27me3
- Stegeman et al. (2016) Interaction between hypoxia, AKT and HIF-1 signaling in HNSCC and NSCLC: implications for future treatment strategies
- Maeda et al. (2016) In Vivo Imaging Reveals Significant Tumor Vascular Dysfunction and Increased Tumor Hypoxia-Inducible Factor-1Î± Expression Induced by High Single-Dose Irradiation in a Pancreatic Tumor Model
- Mysore et al. (2016) A DNA-binding Molecule Targeting the Adaptive Hypoxic Response in Multiple Myeloma Has Potent Antitumor Activity
Hypoxystation users are showing that “culturing cells in ambient air, or ‘normoxia’ is far from physiological.“
Visit HypOxygen at the 15th International Tumor Microenvironment Workshop in Miami