Our American distributor, HypOxygen, are exhibiting at the American Association of Cancer Research annual meeting. Exhibiting the Whitley H35 Hypoxystation and the Whitley i2 Instrument Workstation, HypOxygen will be promoting the importance of using this equipment when working at different stages of cell culture within the context of cancer research. The words below, from HypOxygen, describe this in more detail.
The hallmarks of cancer, from reprogramming energy metabolism through sustained angiogenesis to evasion of the immune response, are linked to the low oxygen tension typical of the tumour microenvironment. Hypoxia is a crucial determinant of cellular signalling, inflammation, cancer progression, and therapy resistance, through pathways mediated by HIF’s and other oxygen-sensing factors. That’s why cancer research consistently benefits from the use of a closed workstation for cell culture that supports in vivo physiology and behaviour. Based on these insights, the Hypoxystation creates conditions that mimic the cellular microenvironment with regard to oxygen tension, temperature, and humidity, while class 3 clean air through HEPA filtration and sterile humidity guarantee worry-free long-term cell culture.
The capacity to stably incubate cell cultures at hypoxic conditions down to 0.1% oxygen depends on precise monitoring of conditions, accurate regulation of gasses, and on the ability to retain the atmosphere for a long time once it is achieved. Based on studies performed at Don Whitley Scientific recently, the “at rest” gas consumption in our H35 Hypoxystation is much lower than in other workstations: Nitrogen 3.5 l/24 hrs, CO2 1 l/24 hours. In contrast, published nitrogen usage in competitors’ hypoxia workstations is 28 and 47 times higher, and CO2 consumption is 5-7 times higher.
You can find the details of that study on the HypOxygen website here.
HypOxygen will be showing the H35 and i2 Instrument Hypoxystations for physiological cell culture at the AACR Annual Meeting in New Orleans from 17/04/16 – 20/04/15 at booth 448.