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Whitley H35 HEPA Hypoxystation

The H35 HEPA Hypoxystation is a cell culture chamber ideal for those requiring HEPA filtration, as well as being able to accurately control oxygen, carbon dioxide, temperature and humidity.

  • Control oxygen in 0.1% increments up to 20%; carbon dioxide in 0.1% increments up to 15%; and relative humidity up to 80% - provides flexibility in your research and confidence in your results.

  • Fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly. Placing the filters inside the workstation prevents the filter becoming saturated.

  • Rather than use off-the-shelf filters, DWS has worked with one of the world's leading filter manufacturers to develop a filter specifically tailored to suit this Hypoxystation.

  • Levels of atmospheric cleanliness inside the workstation exceeds the requirements of ISO 14644 Class 3. We can provide proof of atmospheric cleanliness.

  • Fitted with a fan that circulates the internal atmosphere continuously - contributing to even temperature distribution and ensuring any internal particulate will be in motion.

  • Has a removable front as standard to facilitate thorough cleaning and the transfer of bulk samples or larger pieces of equipment for use in the workstation.

  • Built-in rapid airlock to ensure samples can be transferred into the workstation atmosphere as soon as possible (only takes 60 seconds).

  • Colour, touch-screen control panel for ease of use and for visual display of parameters such as oxygen level, carbon dioxidelevel, temperature, humidity, and airlock cycle status.

  • Fully integrated gas control avoids bulky add-on systems and reduces the need for additional bench space.

  • Carbon dioxide monitoring system fitted as standard to enable you to experiment using different concentrations of carbon dioxide.

An ideal solution for Seahorse Analyzer users is to purchase the H35 HEPA along with the Whitley i2 Instrument Workstation. These two workstations can be connected via the Whitley Transfer Tunnel, enabling preparation of cell lines under hypoxic conditions and their transfer directly into the i2 without exposure to air.

Don Whitley Scientific provides the best possible service and maintenance support for this temperature controlled chamber. Please contact us to request a quotation.

View Specifications


Capacity (Litres) 300
Port/Airlock Capacity 12 litres / Accommodates up to 44 x 96 well plates or 84 x T25 tissue culture flasks
Airlock Size 223mm Deep x 190mm High
Porthole System Manual, oval ports
Gas Supplies CO2, N2, Air
Footswitch Wireless
Auto Sleeve Gassing Available
Internal Mains Socket Available
Storage Trays Available
Single Sample Entry Available
Data Logging Available
Airlock Cycle Time (seconds) 60
Extra Cable Glands Available
Microscope Please contact us for more information
Automatic Dehumidifier Fitted as standard
Automatic Humidifier Available
Removable Front Fitted as standard
Dimensions (w/d/h - mm) 1255 / 810 / 710
Weight (lbs/kg) 264.5 / 120
CO2 Monitoring Fitted as standard
Vacuum Take-off Available
Touchscreen control Yes
HEPA Fitted as standard




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