DON WHITLEY SCIENTIFIC – THE LEADING INTERNATIONAL SUPPLIER TO THE MICROBIOLOGY AND TISSUE CULTURE INDUSTRIES


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Microfluidic Gradient Chip

The Gradient Chip consists of a culture chamber fed by two separate media channels, one at each side of the chamber, allowing shear stress free flow culture in order to generate long term, stable gradient profiles of soluble molecules and dissolved oxygen in 2D and 3D environments. Suitable for use with adherent and non-adherent cells, the Gradient Chip allows for monolayer culture on the bottom of the chamber, or culture in 3D within a hydrogel scaffold. Due to the gradient being created by two different flow circuits, altering the media composition in either of these circuits allows alteration of the gradient perceived by the cells at any point during the experimental process.

As with all our microfluidic chips, the Gradient Chip allows for vast reductions of media and supplement required for experiments, and also has an optical grade glass bottom to allow real time microscopy imaging.

Example applications include:

  1. Chemotaxis of adherent and non-adherent cells

  2. In vitro simulation of a tumour microenvironment

  3. Migration assays

  4. Angiogenic sprouting

  5. Modulation of cell behaviour by oxygen concentration

  6. Axon guidance

(See images and videos for more detail)
 

Technical Specifications

Outer dimensions of the holder: Ø 63mm x 30.5mm

Thickness of the culture chamber: 300µm

Growth surface area of the chamber (2D configuration): 8mm2

Volume of the chamber (3D configuration): 2.4µl

Bottom thickness: 100μm

Catalogue # (chip+holder,sterile,10 units): REF 200.002

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Specifications

Dimensions (w/d/h - mm) Äußere Abmessungen des Halters: 63 / 63 / 30.5

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Gradient Chip Brochure

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